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MHC Class II antibody [HLA-Pan/2967R]

Anti-MHC Class II antibody [HLA-Pan/2967R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02758
Anti-MHC Class II antibody [HLA-Pan/2967R] used in Western Blot (WB). GTX02758
Anti-MHC Class II antibody [HLA-Pan/2967R] used in IHC (Paraffin sections) (IHC-P). GTX02758
Anti-MHC Class II antibody [HLA-Pan/2967R] used in Protein Array (Protein Array). GTX02758
Anti-MHC Class II antibody [HLA-Pan/2967R] used in Flow cytometry (FACS). GTX02758
Anti-MHC Class II antibody [HLA-Pan/2967R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02758

Cat. No. GTX02758

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HLA-Pan/2967R

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all MHC Class II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
ICC/IF 1-2 μg/ml
IHC-P 1-2 μg/ml
FACS 1-2 μg/million cells
ELISA 2-5 μg/ml (for coating)
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

Observed MW

33-35 kDa. The observed M.W. is based on the following publication. PMID: 9931307

Product Note

It reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class II, DP beta 1 , major histocompatibility complex, class II, DQ alpha 1 , major histocompatibility complex, class II, DR alpha , DPB1 , HLA-DP , HLA-DP1B , HLA-DPB , CELIAC1 , DQ-A1 , DQA1 , HLA-DQA , HLA-DRA1

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Research Area

DATA IMAGES

Anti-MHC Class II antibody [HLA-Pan/2967R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02758

GTX02758 ICC/IF Image

ICC/IF analysis of PFA fixed Ramos cells using GTX02758 MHC Class II antibody [HLA-Pan/2967R].
Green : Primary antibody
Red : nuclear counterstain

Anti-MHC Class II antibody [HLA-Pan/2967R] used in Western Blot (WB). GTX02758

GTX02758 WB Image

WB analysis of Ramos whole cell lysate using GTX02758 MHC Class II antibody [HLA-Pan/2967R].

Anti-MHC Class II antibody [HLA-Pan/2967R] used in IHC (Paraffin sections) (IHC-P). GTX02758

GTX02758 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX02758 MHC Class II antibody [HLA-Pan/2967R].

Anti-MHC Class II antibody [HLA-Pan/2967R] used in Protein Array (Protein Array). GTX02758

GTX02758 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA-Pan Rabbit Recombinant Monoclonal Antibody (HLA-Pan/2967R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD') above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD') between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Anti-MHC Class II antibody [HLA-Pan/2967R] used in Flow cytometry (FACS). GTX02758

GTX02758 FACS Image

FACS analysis of Raji cells using GTX02758 MHC Class II antibody [HLA-Pan/2967R].
Blue : Primary antibody
Red : Isotype control

Anti-MHC Class II antibody [HLA-Pan/2967R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02758

GTX02758 ICC/IF Image

ICC/IF analysis of PFA fixed Raji cells using GTX02758 MHC Class II antibody [HLA-Pan/2967R].
Green : Primary antibody
Red : nuclear counterstain

REFERENCE

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REVIEW

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Package List Price ($)
$ 529