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MICA antibody

Anti-MICA antibody used in IHC (Paraffin sections) (IHC-P). GTX16991
Anti-MICA antibody used in Western Blot (WB). GTX16991
Anti-MICA antibody used in IHC (Paraffin sections) (IHC-P). GTX16991

Cat. No. GTX16991

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all MICA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
IHC-P 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

A549 Cell Lysate(GTX27910) , Human Colon Tissue Lysate(GTX27928)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MICA antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MICA.The immunogen is located within the last 50 amino acids of MICA.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MHC class I polypeptide-related sequence A , MIC-A , PERB11.1

Cellular Localization

Cell membrane,Cytoplasm

Background

MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-MICA antibody used in IHC (Paraffin sections) (IHC-P). GTX16991

GTX16991 IHC-P Image

IHC-P analysis of human colon tissue using GTX16991 MICA antibody.
Working concentration : 10 μg/ml

Anti-MICA antibody used in Western Blot (WB). GTX16991

GTX16991 WB Image

WB analysis of A-20 cell lysate using GTX16991 MICA antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-MICA antibody used in IHC (Paraffin sections) (IHC-P). GTX16991

GTX16991 IHC-P Image

IHC-P analysis of human colon tissue using GTX16991 MICA antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399