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MICAL1 antibody [4B1]

Overexpression
Anti-MICAL1 antibody [4B1] used in Western Blot (WB). GTX84121
Anti-MICAL1 antibody [4B1] used in IHC (Paraffin sections) (IHC-P). GTX84121
Anti-MICAL1 antibody [4B1] used in IHC (Paraffin sections) (IHC-P). GTX84121
Anti-MICAL1 antibody [4B1] used in IHC (Paraffin sections) (IHC-P). GTX84121
Anti-MICAL1 antibody [4B1] used in IHC (Paraffin sections) (IHC-P). GTX84121
Anti-MICAL1 antibody [4B1] used in Flow cytometry (FACS). GTX84121
Anti-MICAL1 antibody [4B1] used in IHC (Paraffin sections) (IHC-P). GTX84121
Anti-MICAL1 antibody [4B1] used in Western Blot (WB). GTX84121
Anti-MICAL1 antibody [4B1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84121
Anti-MICAL1 antibody [4B1] used in IHC (Paraffin sections) (IHC-P). GTX84121
Anti-MICAL1 antibody [4B1] used in Flow cytometry (FACS). GTX84121

Cat. No. GTX84121

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4B1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all MICAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human MICAL1 (NP_073602) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated monooxygenase, calponin and LIM domain containing 1 , MICAL , MICAL-1 , NICAL

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

May be a cytoskeletal regulator that connects NEDD9 to intermediate filaments.

Database

Research Area

DATA IMAGES

Anti-MICAL1 antibody [4B1] used in Western Blot (WB). GTX84121

GTX84121 WB Image

WB analysis of various cell lines using GTX84121 MICAL1 antibody [4B1].
Loading : 10 ug per lane
Dilution : 1:200

Anti-MICAL1 antibody [4B1] used in IHC (Paraffin sections) (IHC-P). GTX84121

GTX84121 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84121 MICAL1 antibody [4B1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MICAL1 antibody [4B1] used in IHC (Paraffin sections) (IHC-P). GTX84121

GTX84121 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX84121 MICAL1 antibody [4B1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MICAL1 antibody [4B1] used in IHC (Paraffin sections) (IHC-P). GTX84121

GTX84121 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84121 MICAL1 antibody [4B1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MICAL1 antibody [4B1] used in IHC (Paraffin sections) (IHC-P). GTX84121

GTX84121 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX84121 MICAL1 antibody [4B1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MICAL1 antibody [4B1] used in Flow cytometry (FACS). GTX84121

GTX84121 FACS Image

FACS analysis of Jurkat cells using GTX84121 MICAL1 antibody [4B1].
Red : Primary antibody
Blue : Negative control antibody

Anti-MICAL1 antibody [4B1] used in IHC (Paraffin sections) (IHC-P). GTX84121

GTX84121 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84121 MICAL1 antibody [4B1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MICAL1 antibody [4B1] used in Western Blot (WB). GTX84121

GTX84121 WB Image

Overexpression

WB analysis of HEK293T cells transfected with MICAL1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84121 MICAL1 antibody [4B1].
Loading : 5 ug per lane

Anti-MICAL1 antibody [4B1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84121

GTX84121 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with MICAL1 plasmid using GTX84121 MICAL1 antibody [4B1].

Anti-MICAL1 antibody [4B1] used in IHC (Paraffin sections) (IHC-P). GTX84121

GTX84121 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84121 MICAL1 antibody [4B1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MICAL1 antibody [4B1] used in Flow cytometry (FACS). GTX84121

GTX84121 FACS Image

FACS analysis of HEK293T cells transfected with either MICAL1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84121 MICAL1 antibody [4B1].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519