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MID1IP1 antibody

Anti-MID1IP1 antibody used in Western Blot (WB). GTX106864
Anti-MID1IP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106864
Anti-MID1IP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106864

Cat. No. GTX106864

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MID1IP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MID1IP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950918

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MID1 interacting protein 1 , G12-like , MIG12 , S14R , STRAIT11499 , THRSPL

Cellular Localization

Nucleus , Cytoplasm

Database

Research Area

DATA IMAGES

Anti-MID1IP1 antibody used in Western Blot (WB). GTX106864

GTX106864 WB Image

Sample(30 ug whole cell lysate)
A:293T
B:Hep G2 (GTX27900)
12% SDS PAGE
GTX106864 diluted at 1:1000

Anti-MID1IP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106864

GTX106864 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using MID1IP1(GTX106864) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-MID1IP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106864

GTX106864 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using MID1IP1(GTX106864) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169