GeneTex
United States (US)

MID2 antibody, C-term

GTX14749_325_IHC-P_18121410_169.jpg
GTX14749_1350_WB_18121410_236.jpg

Cat No. GTX14749

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 5-10μg/ml

Note :

IHC-P
Human Kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules.

Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX14749-PEP).

Specificity/Sensitivity

This antibody is expected to recognise both human isoforms of this protein.

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence QESPYVSGMKTCH, from the C Terminus of the protein sequence according to NP_036348.2; NP_438112.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Midline 2,Fxy2,Mrx101,Rnf60,Trim1,Mid2

Cellular Localization

Cytoplasmic

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES
GTX14749_325_IHC-P_18121410_169.jpg

GTX14749 IHC-P Image

IHC-P analysis of human kidney using GTX14749 MID2 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5µg/ml

GTX14749_1350_WB_18121410_236.jpg

GTX14749 WB Image

WB analysis of mouse (A) and rat (B) heart, and rat kidney (C) lysates using GTX14749 MID2 antibody, C-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329