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MID2 antibody, C-term

Anti-MID2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX14749
Anti-MID2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX14749

Cat. No. GTX14749

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all MID2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 5-10μg/ml

Note :

IHC-P
Human Kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules.
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX14749-PEP).

Product Note

This antibody is expected to recognise both human isoforms of this protein.

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence QESPYVSGMKTCH, from the C Terminus of the protein sequence according to NP_036348.2; NP_438112.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

midline 2 , FXY2 , MRX101 , RNF60 , TRIM1

Cellular Localization

Cytoplasm , Cytoplasm, cytoskeleton

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-MID2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX14749

GTX14749 IHC-P Image

IHC-P analysis of human kidney tissue using GTX14749 MID2 antibody, C-term antibody.
Antigen retrieval: Heat-induced antigen retrieval with citrate buffer pH 6
Dilution: 8μg/ml

Anti-MID2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX14749

GTX14749 IHC-P Image

IHC-P analysis of human kidney tissue with no primary antibody (negative control).

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399