GeneTex
United States (US)

MID2 antibody [C3], C-term

GTX106442_WB_19011506_392.jpg

Cat No. GTX106442

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

MID2 tansfected 293T cell

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MID2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ37715 antibody, FLJ41813 antibody, FXY2 antibody, RNF60 antibody, TRIM1 antibody, MID2 antibody, tripartite motif-containing protein 1 antibody, RING finger protein 60 antibody, tripartite motif protein 1 antibody, midline defect 2 antibody, midin 2 antibody, midline-2 antibody, probable E3 ubiquitin-protein ligase MID2 antibody, midin-2 antibody, midline 2 antibody

Cellular Localization

Cytoplasm , cytoskeleton

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106442_WB_19011506_392.jpg

GTX106442 WB Image

Western Blot analysis of MID2 expression in transfected 293T cell line by MID2 polyclonal antibody.

A: Non-transfected lysate.

B: MID2 transfected lysate.

12% SDS PAGE

GTX106442 diluted at 1:5000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169