GeneTex

  • Country / Location Selection

United States (US)

MIF antibody

Anti-MIF antibody used in Western Blot (WB). GTX48478
Anti-MIF antibody used in Western Blot (WB). GTX48478

Cat. No. GTX48478

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all MIF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:1000-1:5000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to KLH.

Purification

IgY fraction
This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

macrophage migration inhibitory factor , GIF , GLIF , MMIF

Cellular Localization

Secreted,Cytoplasm

Background

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

Database

Research Area

DATA IMAGES

Anti-MIF antibody used in Western Blot (WB). GTX48478

GTX48478 WB Image

Western blot analysis of GeneTex's IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 μg of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kDa. A 4-20% gradient gel was used to separate the proteins by SDS-PAGE. The protein was transferred to nitro-cellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye800 conjugated Gt-a-Chicken Rabbit IgG [H&L] for 1 h at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Anti-MIF antibody used in Western Blot (WB). GTX48478

GTX48478 WB Image

WB analysis of various samples using GTX48478 MIF antibody.
Lane 1 : Mock
Lane 2 : recombinant human MIF protein
Loading : 100 ng

REFERENCE

There are currently no references for MIF antibody (GTX48478). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for MIF antibody (GTX48478). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399