APPLICATION
Application Note
We recommend using the capture antibody at a concentration of 5μg/mL and the detection antibody at a concentration of 1.5μg/mL. Optimal dilutions should be determined experimentally by the researcher.
PROPERTIES
Form
Liquid
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Macrophage Migration Inhibitory Factor , Gif , Glif , Mmif , Mif
Background
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq]
Research Area
DATA IMAGES
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GTX500014 ELISA Image
ELISA detection of MIF using GTX101162 for capture at a concentration of 5 μg/mL and GTX89535 for detection at a concentration of 1.5 μg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.
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REFERENCE
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REVIEW
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