MIF antibody [2Ar3]

Cat No. GTX14575

Host	Mouse
Clonality	Monoclonal
Clone Name	2Ar3
Isotype	IgG1
Application	WB, ELISA
Reactivity	Human

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Datasheet

APPLICATION

Application Note

For ELISA: Use at a concentration of 4 μg/ml. For WB: Use at a concentration of 1 - 2 μg/ml. Detection limit is ~5 ng/ml under non-reducing and reducing conditions. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

12 kDa. ( Note )

Specificity/Sensitivity

Recognizes recombinant and natural human Migration Inhibitory Factor (MIF). Based on direct ELISA, shows no crossreactivity with most cytokines.

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 5% trehalose

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E. coli-derived recombinant full length protein (Human).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Macrophage Migration Inhibitory Factor,Gif,Glif,Mmif,Mif

Background

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.