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MIF antibody [4E4]

Anti-MIF antibody [4E4] used in Western Blot (WB). GTX53741

Cat. No. GTX53741

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E4

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all MIF products

APPLICATION

Application Note

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human MIF (1-114 aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

macrophage migration inhibitory factor , GIF , GLIF , MMIF

Cellular Localization

Secreted , Cytoplasm

Background

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MIF antibody [4E4] used in Western Blot (WB). GTX53741

GTX53741 WB Image

WB analysis of HL-60 lysate using MIF antibody at a dilution of 1:1,000.

REFERENCE

Application Reference

Y Wei et al. PLoS One 2023; 18 (8) : e0288281 Human embryonic stem cells secrete macrophage migration inhibitory factor: A novel finding
Application : ICC/IF
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399