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MIF antibody [4E4]

Anti-MIF antibody [4E4] used in Western Blot (WB). GTX53741

Cat. No. GTX53741

Host

Mouse

Clonality

Monoclonal

Clone Name

4E4

Isotype

IgG1

Application

WB, ICC/IF, FACS, ELISA

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human MIF (1-114 aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

macrophage migration inhibitory factor , GIF , GLIF , MMIF

Cellular Localization

Secreted , Cytoplasm

Background

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MIF antibody [4E4] used in Western Blot (WB). GTX53741

GTX53741 WB Image

WB analysis of HL-60 lysate using MIF antibody at a dilution of 1:1,000.

REFERENCE

There are currently no references for MIF antibody [4E4] (GTX53741). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for MIF antibody [4E4] (GTX53741). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399