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MIF antibody, C-term

Anti-MIF antibody, C-term used in Western Blot (WB). GTX89535
Anti-MIF antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89535
Anti-MIF antibody, C-term used in Western Blot (WB). GTX89535
Anti-MIF antibody, C-term used in ELISA (ELISA). GTX89535

Cat. No. GTX89535

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human
Package
100 μg ($399)
See all MIF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01-0.03μg/ml
IHC-P 5μg/ml
ELISA Assay dependent
Sandwich ELISA Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89535-PEP).

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NAANVGWNNSTFA, from the C Terminus of the protein sequence according to NP_002406.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

macrophage migration inhibitory factor , GIF , GLIF , MMIF

Cellular Localization

Secreted , Cytoplasm

Background

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MIF antibody, C-term used in Western Blot (WB). GTX89535

GTX89535 WB Image

WB analysis of Daudi (A) and Jurkat (B) lysate using GTX89535 MIF antibody, C-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

Anti-MIF antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89535

GTX89535 IHC-P Image

IHC-P analysis of human thymus using GTX89535 MIF antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-MIF antibody, C-term used in Western Blot (WB). GTX89535

GTX89535 WB Image

WB analysis of human thymus lysate using GTX89535 MIF antibody, C-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

Anti-MIF antibody, C-term used in ELISA (ELISA). GTX89535

GTX89535 ELISA Image

ELISA analysis of MIF protein using GTX89535 MIF antibody, C-term.
Capture : GTX101162 (5μg/ml)
Detection : GTX89535 (1.5μg/ml)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399