APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.01-0.03μg/ml |
| 5μg/ml |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89535-PEP).
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS pH7.3, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-NAANVGWNNSTFA, from the C Terminus of the protein sequence according to NP_002406.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
macrophage migration inhibitory factor , GIF , GLIF , MMIF
Cellular Localization
Secreted , Cytoplasm
Background
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89535 WB Image
WB analysis of human thymus lysate using GTX89535 MIF antibody, C-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89535 IHC-P Image
IHC-P analysis of human thymus using GTX89535 MIF antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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GTX89535 ELISA Image
ELISA analysis of MIF protein using GTX89535 MIF antibody, C-term.
Capture : GTX101162 (5μg/ml)
Detection : GTX89535 (1.5μg/ml)
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GTX89535 WB Image
WB analysis of Daudi (A) and Jurkat (B) lysate using GTX89535 MIF antibody, C-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for MIF antibody, C-term (GTX89535). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MIF antibody, C-term (GTX89535). Be the first to share your experience with this product.
