Indirect ELISA analysis was performed by coating the plate with recombinant Mammalian cell expressed, full-length human MIF protein (96.85-1.51 nM). Coated protein was probed with MIF antibody [HL2964] (GTX640351) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [HL2964] (GTX640351) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [HL2964] (GTX640351) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.