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MIF antibody [N1C3]

Anti-MIF antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101162
Anti-MIF antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101162
Anti-MIF antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101162
Anti-MIF antibody [N1C3] used in Western Blot (WB). GTX101162
Anti-MIF antibody [N1C3] used in Western Blot (WB). GTX101162
Anti-MIF antibody [N1C3] used in ELISA (ELISA). GTX101162

Cat No. GTX101162

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA, sELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
sELISA Assay dependent

Note :

sELISA
Capture: GTX101162 , Detection: GTX89535

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Molt-4 , mouse brain

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

macrophage migration inhibitory factor , GIF , GLIF , MMIF

Background

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MIF antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101162

GTX101162 IHC-P Image

MIF antibody [N1C3] detects MIF protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MIF antibody [N1C3] (GTX101162) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MIF antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101162

GTX101162 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MIF(GTX101162) antibody at 1:200 dilution.

Anti-MIF antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101162

GTX101162 IHC-P Image

MIF antibody [N1C3] detects MIF protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MIF antibody [N1C3] (GTX101162) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MIF antibody [N1C3] used in Western Blot (WB). GTX101162

GTX101162 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MIF antibody [N1C3] used in Western Blot (WB). GTX101162

GTX101162 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
15% SDS PAGE
GTX101162 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MIF antibody [N1C3] used in ELISA (ELISA). GTX101162

GTX101162 ELISA Image

ELISA detection of MIF using GTX101162 for capture at a concentration of 5 μg/mL and GTX89535 for detection at a concentration of 1.5 μg/mL.

REFERENCE

REVIEW

MIF antibody [N1C3] Cat No. GTX101162

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 25-Jul-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1299 cells
Amount used : 30μg
Blocking : milk, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf