MIF antibody [N1C3]

Name: MIF antibody [N1C3]
Brand: GeneTex
SKU: GTX101162
Price: 329.00 USD
Rating: 4 (1 reviews)

Anti-MIF antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101162

Anti-MIF antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101162

Anti-MIF antibody [N1C3] used in Western Blot (WB). GTX101162

Anti-MIF antibody [N1C3] used in ELISA (ELISA). GTX101162

Cat No. GTX101162

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($329), 25 μl ($169)

See all MIF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
ELISA	1:1000-1:10000
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture: GTX101162 , Detection: GTX89535

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Molt-4 , mouse brain

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

macrophage migration inhibitory factor , GIF , GLIF , MMIF

Background

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq]

Database

Gene ID: 4282 MIF
UniProt: P14174 MIF

Research Area

DATA IMAGES

GTX101162 IHC-P Image

MIF antibody [N1C3] detects MIF protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MIF antibody [N1C3] (GTX101162) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101162 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MIF(GTX101162) antibody at 1:200 dilution.

GTX101162 IHC-P Image

MIF antibody [N1C3] detects MIF protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MIF antibody [N1C3] (GTX101162) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101162 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX101162 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
15% SDS PAGE
GTX101162 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101162 ELISA Image

ELISA detection of MIF using GTX101162 for capture at a concentration of 5 μg/mL and GTX89535 for detection at a concentration of 1.5 μg/mL.

REFERENCE

Application Reference

Yulizal OK et al. J Adv Vet Anim Res 2020; 7 (2) : 209-217 The effect of snakehead fish extract supplementation to first-line eradication regimen on macrophage migration inhibitory factor (MIF) expression in rats induced by Helicobacter pylori infection.

Application : IHC-P

Reactivity : Rat

Movila A et al. J Bone Miner Res 2016; (Epub) Macrophage Migration Inhibitory Factor (MIF) Supports Homing of Osteoclast Precursors to Peripheral Osteolytic Lesions.

Application : IHC-P

Reactivity : Mouse

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