MIF antibody [N1C3]

Name: MIF antibody [N1C3]
Brand: GeneTex
SKU: GTX101162
Price: 399.00 USD
Rating: 4.5 (2 reviews)

Anti-MIF antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101162

Anti-MIF antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101162

Anti-MIF antibody [N1C3] used in Western Blot (WB). GTX101162

Anti-MIF antibody [N1C3] used in ELISA (ELISA). GTX101162

Cat. No. GTX101162

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all MIF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
ELISA	1:1000-1:10000
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture: GTX101162 , Detection: GTX89535

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Molt-4 , mouse brain

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950920

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

macrophage migration inhibitory factor , GIF , GLIF , MMIF

Background

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq]

Database

Gene ID: 4282 MIF
UniProt: P14174 MIF

Research Area

DATA IMAGES

GTX101162 IHC-P Image

MIF antibody [N1C3] detects MIF protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MIF antibody [N1C3] (GTX101162) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101162 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MIF(GTX101162) antibody at 1:200 dilution.

GTX101162 IHC-P Image

MIF antibody [N1C3] detects MIF protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MIF antibody [N1C3] (GTX101162) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101162 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX101162 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
15% SDS PAGE
GTX101162 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101162 ELISA Image

ELISA detection of MIF using GTX101162 for capture at a concentration of 5 μg/mL and GTX89535 for detection at a concentration of 1.5 μg/mL.

REFERENCE

Application Reference

Murata R et al. Pharmaceutics 2022; 14 (3) Long-Acting Thioredoxin Ameliorates Doxorubicin-Induced Cardiomyopathy via Its Anti-Oxidative and Anti-Inflammatory Action.

Application : WB

Reactivity : Mouse

Chen LL et al. Evid Based Complement Alternat Med 2021; 2021 : 5205759 Suppression of Inflammatory and Fibrotic Signals by Cinnamon (Cinnamomum cassia) and Cinnamaldehyde in Cyclophosphamide-Induced Overactive Bladder in Mice.

Application : IHC-Fr

Reactivity : Mouse

Yulizal OK et al. J Adv Vet Anim Res 2020; 7 (2) : 209-217 The effect of snakehead fish extract supplementation to first-line eradication regimen on macrophage migration inhibitory factor (MIF) expression in rats induced by Helicobacter pylori infection.

Application : IHC-P

Reactivity : Rat

Movila A et al. J Bone Miner Res 2016; (Epub) Macrophage Migration Inhibitory Factor (MIF) Supports Homing of Osteoclast Precursors to Peripheral Osteolytic Lesions.

Application : IHC-P

Reactivity : Mouse

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