MIF antibody

Cat. No. GTX48478
Cat. No.  GTX48478
Anti-MIF antibody used in Western Blot (WB). GTX48478
GTX48478 WB Image

WB analysis of various samples using GTX48478 MIF antibody.
Lane 1 : Mock
Lane 2 : recombinant human MIF protein
Loading : 100 ng

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Anti-MIF antibody used in Western Blot (WB). GTX48478
GTX48478 WB Image

Western blot analysis of GeneTex's IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 μg of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kDa. A 4-20% gradient gel was used to separate the proteins by SDS-PAGE. The protein was transferred to nitro-cellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye800 conjugated Gt-a-Chicken Rabbit IgG [H&L] for 1 h at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

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Anti-MIF antibody used in Western Blot (WB). GTX48478
Anti-MIF antibody used in Western Blot (WB). GTX48478
  • Host
    Chicken
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Applications
    WB ELISA
  • Reactivity
    Human
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