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MIG antibody

Orthogonal
Anti-MIG antibody used in Western Blot (WB). GTX108422
Anti-MIG antibody used in IHC (Paraffin sections) (IHC-P). GTX108422
Anti-MIG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108422
Anti-MIG antibody used in IHC (Paraffin sections) (IHC-P). GTX108422

Cat. No. GTX108422

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MIG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MIG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950076

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine ligand 9 , CMK , Humig , MIG , SCYB9 , crg-10

Cellular Localization

Secreted

Background

The function of this gene has not been specifically defined; however, it is thought to be involved in T cell trafficking. This gene has been localized to 4q21 with INP10, which is also a member of the chemokine family of cytokines. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MIG antibody used in Western Blot (WB). GTX108422

GTX108422 WB Image

Orthogonal

Untreated (–) and treated (+) MBA-MD-231 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIG antibody (GTX108422) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MIG antibody used in IHC (Paraffin sections) (IHC-P). GTX108422

GTX108422 IHC-P Image

MIG antibody detects CXCL9 protein at cytosol on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded lung adenocarcinoma.
MIG antibody (GTX108422) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MIG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108422

GTX108422 ICC/IF Image

MIG antibody detects MIG protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 5 min.
Green: MIG protein stained by MIG antibody (GTX108422) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-MIG antibody used in IHC (Paraffin sections) (IHC-P). GTX108422

GTX108422 IHC-P Image

MIG antibody detects MIG protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
MIG stained by MIG antibody (GTX108422) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169