GeneTex
United States (US)

MIP1 alpha antibody

Cat No. GTX10381

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA, Neut
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Neut Assay dependent
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

Specificity/Sensitivity

The antibody will neutralize the biological activity of recombinant human, but not mouse, MIP-1α.
PROPERTIES

Form

Liquid

Buffer

PBS PH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant, human macrophage inflammatory protein-1alpha, expressed in E. coli

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-C Motif Chemokine Ligand 3,G0S19-1,Ld78Alpha,Mip-1-Alpha,Mip1A,Scya3,Ccl3

Background

MIP1 alpha belongs to the chemokine beta family. In vitro MIP1 alpha stimulates H2O2 production in human neutrophils. MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor 1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.

Database

Research Area

Package List Price ($)
$ 289