GeneTex
United States (US)

MIPEP antibody [N2C1], Internal

Anti-MIPEP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX105574
Anti-MIPEP antibody [N2C1], Internal used in Western Blot (WB). GTX105574
Anti-MIPEP antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105574

Cat No. GTX105574

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

A431 , HepG2

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIPEP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitochondrial Intermediate Peptidase,Coxpd31,Hmip,Mip,Mipep

Cellular Localization

Mitochondrion matrix

Background

The product of this gene performs the final step in processing a specific class of nuclear-encoded proteins targeted to the mitochondrial matrix or inner membrane. This protein is primarily involved in the maturation of oxidative phosphorylation (OXPHOS)-related proteins. This gene may contribute to the functional effects of frataxin deficiency and the clinical manifestations of Friedreich ataxia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-MIPEP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX105574

GTX105574 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using MIPEP(GTX105574) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MIPEP antibody [N2C1], Internal used in Western Blot (WB). GTX105574

GTX105574 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX105574 diluted at 1:1000

Anti-MIPEP antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105574

GTX105574 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using MIPEP(GTX105574) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169