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MIPEP antibody [N2C1], Internal

Anti-MIPEP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX105574
Anti-MIPEP antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105574
Anti-MIPEP antibody [N2C1], Internal used in Western Blot (WB). GTX105574

Cat. No. GTX105574

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all MIPEP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIPEP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037436

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial intermediate peptidase , COXPD31 , HMIP , MIP

Cellular Localization

Mitochondrion matrix

Background

The product of this gene performs the final step in processing a specific class of nuclear-encoded proteins targeted to the mitochondrial matrix or inner membrane. This protein is primarily involved in the maturation of oxidative phosphorylation (OXPHOS)-related proteins. This gene may contribute to the functional effects of frataxin deficiency and the clinical manifestations of Friedreich ataxia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MIPEP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX105574

GTX105574 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using MIPEP(GTX105574) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MIPEP antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105574

GTX105574 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using MIPEP(GTX105574) antibody at 1:200 dilution.

Anti-MIPEP antibody [N2C1], Internal used in Western Blot (WB). GTX105574

GTX105574 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX105574 diluted at 1:1000

REFERENCE

Application Reference

Jacques S et al. Genetics 2020; 214 (4) : 1103-1120 Imipridone Anticancer Compounds Ectopically Activate the ClpP Protease and Represent a New Scaffold for Antibiotic Development.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169