GeneTex
United States (US)

MIPP antibody [C1C3]

GTX106186_IHC_19011506_448.jpg
GTX106186_WB_19011506_674.jpg
GTX106186_40142_20150529_IHC_M_19011506_686.jpg

Cat No. GTX106186

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , Raji

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIPP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp564L2016 antibody, HIPER1 antibody, MINPP2 antibody, MIPP antibody, MINPP1 antibody, "multiple inositol polyphosphate histidine phosphatase, 1 antibody", "inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase antibody", multiple inositol polyphosphate phosphatase 2 antibody, "ins(1,3,4,5)P(4) 3-phosphatase antibody", multiple inositol polyphosphate phosphatase 1 antibody, multiple inositol-polyphosphate phosphatase 1 antibody

Cellular Localization

Endoplasmic reticulum lumen

Background

MINPP1 hydrolyzes the abundant metabolites inositol pentakisphosphate and inositol hexakisphosphate and, like PTEN (MIM 601728), has the ability to remove 3-phosphate from inositol phosphate substrates.[supplied by OMIM]
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Database

Research Area

DATA IMAGES
GTX106186_IHC_19011506_448.jpg

GTX106186 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using MIPP (GTX106186) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106186_WB_19011506_674.jpg

GTX106186 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX106186 diluted at 1:1000

GTX106186_40142_20150529_IHC_M_19011506_686.jpg

GTX106186 IHC-P Image

MIPP antibody [C1C3] detects MIPP protein at cytoplasm in mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
MIPP antibody [C1C3] (GTX106186) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf