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MIPP antibody [N2C3]

Anti-MIPP antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108680
Anti-MIPP antibody [N2C3] used in Western Blot (WB). GTX108680
Anti-MIPP antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108680
Anti-MIPP antibody [N2C3] used in Western Blot (WB). GTX108680

Cat. No. GTX108680

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MIPP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

Mouse brain , Molt-4

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIPP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950922

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

multiple inositol-polyphosphate phosphatase 1 , HIPER1 , MINPP2 , MIPP

Cellular Localization

Endoplasmic reticulum lumen

Background

MINPP1 hydrolyzes the abundant metabolites inositol pentakisphosphate and inositol hexakisphosphate and, like PTEN (MIM 601728), has the ability to remove 3-phosphate from inositol phosphate substrates.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-MIPP antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108680

GTX108680 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft , using MIPP (GTX108680) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MIPP antibody [N2C3] used in Western Blot (WB). GTX108680

GTX108680 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX108680 diluted at 1:1000

Anti-MIPP antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108680

GTX108680 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using MIPP(GTX108680) antibody at 1:200 dilution.

Anti-MIPP antibody [N2C3] used in Western Blot (WB). GTX108680

GTX108680 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX108680 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169