GeneTex
United States (US)

MIPP antibody [N2C3]

GTX108680_40044_WB_M_Brain_19011507_346.jpg
GTX108680_40044_IFA_19011507_302.jpg
GTX108680_IHC_19011507_864.jpg
GTX108680_WB_19011507_885.jpg

Cat No. GTX108680

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIPP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Multiple Inositol-Polyphosphate Phosphatase 1,Hiper1,Minpp2,Mipp,Minpp1

Cellular Localization

Endoplasmic reticulum lumen

Background

MINPP1 hydrolyzes the abundant metabolites inositol pentakisphosphate and inositol hexakisphosphate and, like PTEN (MIM 601728), has the ability to remove 3-phosphate from inositol phosphate substrates.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX108680_40044_WB_M_Brain_19011507_346.jpg

GTX108680 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX108680 diluted at 1:1000

GTX108680_40044_IFA_19011507_302.jpg

GTX108680 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using MIPP(GTX108680) antibody at 1:200 dilution.

GTX108680_IHC_19011507_864.jpg

GTX108680 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft , using MIPP (GTX108680) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108680_WB_19011507_885.jpg

GTX108680 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX108680 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169