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MIS12 antibody

Anti-MIS12 antibody used in Immunoprecipitation (IP). GTX130138
Anti-MIS12 antibody used in Western Blot (WB). GTX130138

Cat No. GTX130138

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HeLa , HepG2

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIS12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MIS12 kinetochore complex component , 2510025F08Rik , KNTC2AP , MTW1 , hMis12

Cellular Localization

Nucleus , Kinetochore

Background

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis.

Database

Research Area

DATA IMAGES

Anti-MIS12 antibody used in Immunoprecipitation (IP). GTX130138

GTX130138 IP Image

Immunoprecipitation of MIS12 protein from 293T whole cell extracts using 5 μg of MIS12 antibody (GTX130138).
Western blot analysis was performed using MIS12 antibody (GTX130138).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-MIS12 antibody used in Western Blot (WB). GTX130138

GTX130138 WB Image

MIS12 antibody detects MIS12 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MIS12 antibody (GTX130138) diluted at a dilution of 1:1000.

REFERENCE

There are currently no references for MIS12 antibody (GTX130138). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169