APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2 μg/ml |
1-2 μg/ml |
1-2 μg/million cells |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
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Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
We do not recommend use of this product for Mouse,Rat samples.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
200 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human MiTF protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
melanocyte inducing transcription factor , CMM8 , COMMAD , MI , WS2 , WS2A , bHLHe32
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
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GTX02674 IHC-P Image
IHC-P analysis of human melanoma section using GTX02674 MITF antibody [MITF/2987R].
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GTX02674 IHC-P Image
IHC-P analysis of human melanoma section using GTX02674 MITF antibody [MITF/2987R].
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REFERENCE
There are currently no references for MITF antibody [MITF/2987R] (GTX02674). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MITF antibody [MITF/2987R] (GTX02674). Be the first to share your experience with this product.