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MITF antibody [N2C1], Internal

Anti-MITF antibody [N2C1], Internal used in Western Blot (WB). GTX113776
Anti-MITF antibody [N2C1], Internal used in Western Blot (WB). GTX113776

Cat. No. GTX113776

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MITF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

HeLa , mouse skin

Predict Reactivity

Rat, Bovine, Dog, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MITF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724483

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

melanocyte inducing transcription factor , CMM8 , COMMAD , MI , WS2 , WS2A , bHLHe32

Background

This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MITF antibody [N2C1], Internal used in Western Blot (WB). GTX113776

GTX113776 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX113776 diluted at 1:2000

Anti-MITF antibody [N2C1], Internal used in Western Blot (WB). GTX113776

GTX113776 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MITF antibody [N2C1], Internal (GTX113776) diluted at 1:500.

REFERENCE

Application Reference

Gunarta IK et al. Cancer Sci 2017; 108 (8) : 1602-1611 Critical role of glioma-associated oncogene homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells.
Application : WB
Reactivity : Mouse
Wang Y et al. Oncogene 2016; Androgen receptor promotes melanoma metastasis via altering the miRNA-539-3p/USP13/MITF/AXL signals.
Application : WB
Reactivity : Human
Schlegel NC et al. Exp Dermatol 2014; PI3K signalling is required for a TGFβ-induced epithelial-mesenchymal-like transition (EMT-like) in human melanoma cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169