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MITF antibody [SPM290]

Anti-MITF antibody [SPM290] used in IHC (Paraffin sections) (IHC-P). GTX34843

Cat. No. GTX34843

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM290

Isotype

 IgG1

Application

 IHC-P

Reactivity

 Human
Package
100 μg ($529)
See all MITF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1-2μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NH2 terminus fragment of human Mi protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CMM8 , MI , MITF , WS2 , WS2A , bHLHe32 , melanogenesis associated transcription factor , MiTF , melanocyte inducing transcription factor

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-MITF antibody [SPM290] used in IHC (Paraffin sections) (IHC-P). GTX34843

GTX34843 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX34843 MITF antibody [SPM290].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529