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MITF antibody

KO/KD
Anti-MITF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00738
Anti-MITF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00738
Anti-MITF antibody used in IHC (Frozen sections) (IHC-Fr). GTX00738
Anti-MITF antibody used in Western Blot (WB). GTX00738

Cat. No. GTX00738

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, ChIP assay

Reactivity

 Human, Mouse, Chicken, Xenopus
Package
100 μl ($399)
See all MITF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
ICC/IF Assay dependent
IHC-P 1:300-1:1000
IHC-Fr 1:300-1:1000
ChIP assay 1:200
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Product Note

This antibody recognizes MiTF-M, MiTF-A, MiTF-C, MiTF-H and other MiTF isoforms.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-size human Mitf (isoform M) protein with His tag

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

melanocyte inducing transcription factor , CMM8 , COMMAD , MI , WS2 , WS2A , bHLHe32

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-MITF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00738

GTX00738 ICC/IF Image

ICC/IF analysis of Mouse primary melanocytes (6 weeks) using GTX00738 MITF antibody.
Dilution : 1:500

Anti-MITF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00738

GTX00738 ICC/IF Image

ICC/IF analysis of mouse melanoma B16 cells using GTX00738 MITF antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000
Fixation : 4% PFA
Permeabilization : 0.25 % Triton X-100

Anti-MITF antibody used in IHC (Frozen sections) (IHC-Fr). GTX00738

GTX00738 IHC-Fr Image

IHC-Fr analysis of chick embryo (stage 14, 8 μm) using GTX00738 MITF antibody.
At stage 14, Mitif protein is detected throughout the RPE (Retinal Pigment Epithelium).
Dilution : 1:300
Fixation : 4% PFA

Anti-MITF antibody used in Western Blot (WB). GTX00738

GTX00738 WB Image

KO/KD

WB analysis of various samples using GTX00738 MITF antibody.
Untreared : human melanoma cells
Control : human melanoma cells treated with control siRNA
Mitf : human melanoma cells treated with Mitf siRNA
Dilution : 1:1000

REFERENCE

Application Reference

Hseu YC et al. Redox Biol 2021; 44 : 102007 The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399