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MITF antibody

Anti-MITF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00738
Anti-MITF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00738
Anti-MITF antibody used in Western Blot (WB). GTX00738
Anti-MITF antibody used in IHC (Frozen sections) (IHC-Fr). GTX00738

Cat No. GTX00738

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, ChIP assay

Reactivity

Human, Mouse, Chicken, Xenopus
Package
100 μl ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:3000
ICC/IF Assay dependent
IHC-P 1:300-1:1000
IHC-Fr 1:300-1:1000
ChIP assay 1:200
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes MiTF-M, MiTF-A, MiTF-C, MiTF-H and other MiTF isoforms.

PROPERTIES

Form

Liquid

Buffer

Serum with 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-size human Mitf (isoform M) protein with His tag

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

melanocyte inducing transcription factor , CMM8 , COMMAD , MI , WS2 , WS2A , bHLHe32

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-MITF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00738

GTX00738 ICC/IF Image

ICC/IF analysis of Mouse primary melanocytes (6 weeks) using GTX00738 MITF antibody.
Dilution : 1:500

Anti-MITF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00738

GTX00738 ICC/IF Image

ICC/IF analysis of mouse melanoma B16 cells using GTX00738 MITF antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000
Fixation : 4% PFA
Permeabilization : 0.25 % Triton X-100

Anti-MITF antibody used in Western Blot (WB). GTX00738

GTX00738 WB Image

WB analysis of various samples using GTX00738 MITF antibody.
Untreared : human melanoma cells
Control : human melanoma cells treated with control siRNA
Mitf : human melanoma cells treated with Mitf siRNA
Dilution : 1:1000

Anti-MITF antibody used in IHC (Frozen sections) (IHC-Fr). GTX00738

GTX00738 IHC-Fr Image

IHC-Fr analysis of chick embryo (stage 14, 8 μm) using GTX00738 MITF antibody.
At stage 14, Mitif protein is detected throughout the RPE (Retinal Pigment Epithelium).
Dilution : 1:300
Fixation : 4% PFA

REFERENCE

There are currently no references for MITF antibody (GTX00738). Be the first to share your publications with this product.

REVIEW

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SDS
Sodium Azide.pdf