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MKLP1 antibody

Anti-MKLP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03456
Anti-MKLP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03456
Anti-MKLP1 antibody used in Western Blot (WB). GTX03456
Anti-MKLP1 antibody used in Western Blot (WB). GTX03456

Cat. No. GTX03456

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all MKLP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Dog, Chicken, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human KIF23(Accession Q02241), corresponding to amino acid residues A445-E466.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kinesin family member 23 , CHO1 , KNSL5 , MKLP-1 , MKLP1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-MKLP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03456

GTX03456 ICC/IF Image

ICC/IF analysis of PFA-fixed A549 cells using GTX03456 MKLP1 antibody.
Permeabilization : 0.1% Triton X-100
Red : Primary antibody
Green : beta tubulin
Blue : DAPI

Anti-MKLP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03456

GTX03456 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX03456 MKLP1 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-MKLP1 antibody used in Western Blot (WB). GTX03456

GTX03456 WB Image

WB analysis of MKLP1 transfected 293T cell lysate using GTX03456 MKLP1 antibody. The lane on the left was treated with the antigen-specific peptide.

Anti-MKLP1 antibody used in Western Blot (WB). GTX03456

GTX03456 WB Image

WB analysis of HeLa whole cell lysate using GTX03456 MKLP1 antibody. The lane on the left was treated with blocking peptide.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399