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MKRN1 antibody [N2N3]

Anti-MKRN1 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX106459
Anti-MKRN1 antibody [N2N3] used in Western Blot (WB). GTX106459
Anti-MKRN1 antibody [N2N3] used in Western Blot (WB). GTX106459

Cat. No. GTX106459

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($419),
25 μl ($169)
See all MKRN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A431 , H1299 , HepG2 , rat skin

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MKRN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950923

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

makorin ring finger protein 1 , RNF61

Background

The Makorin ring finger protein-1 gene (MKRN1) is a highly transcribed, intron-containing source for a family of intronless mammalian genes encoding a novel class of zinc finger proteins. Phylogenetic analyses indicate that the MKRN1 gene is the ancestral founder of this gene family (Gray et al., 2000 [PubMed 10843807]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-MKRN1 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX106459

GTX106459 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using MKRN1(GTX106459) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MKRN1 antibody [N2N3] used in Western Blot (WB). GTX106459

GTX106459 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hep G2 (GTX27900)
10% SDS PAGE
MKRN1 antibody
GTX106459 diluted at 1:1000

Anti-MKRN1 antibody [N2N3] used in Western Blot (WB). GTX106459

GTX106459 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MKRN1 antibody [N2N3] (GTX106459) diluted at 1:500.

REFERENCE

Application Reference

Kotla S et al. Metabolism 2019; : 153962 Endothelial senescence-associated secretory phenotype (SASP) is regulated by Makorin-1 ubiquitin E3 ligase.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169