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MKRN2 antibody

Anti-MKRN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115731
Anti-MKRN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115731
Anti-MKRN2 antibody used in Western Blot (WB). GTX115731

Cat. No. GTX115731

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MKRN2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MKRN2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10728911

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

makorin ring finger protein 2 , HSPC070 , RNF62

Background

Members of the makorin family, including MKRN2, have a characteristic zinc finger composition that suggests that they are ribonucleoproteins (Gray et al., 2001 [PubMed 11597136]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-MKRN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115731

GTX115731 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using MKRN2(GTX115731) antibody at 1:500 dilution.

Anti-MKRN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115731

GTX115731 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using MKRN2(GTX115731) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MKRN2 antibody used in Western Blot (WB). GTX115731

GTX115731 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
10% SDS PAGE
GTX115731 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169