GeneTex
United States (US)

MKRN2 antibody

GTX115731_40401_IFA_18121410_129.jpg
GTX115731_40401_IHC_18121410_903.jpg
GTX115731_40401_WB_18121410_818.jpg

Cat No. GTX115731

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MKRN2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Makorin Ring Finger Protein 2,Hspc070,Rnf62,Mkrn2

Background

Members of the makorin family, including MKRN2, have a characteristic zinc finger composition that suggests that they are ribonucleoproteins (Gray et al., 2001 [PubMed 11597136]).[supplied by OMIM]

Database

DATA IMAGES
GTX115731_40401_IFA_18121410_129.jpg

GTX115731 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using MKRN2(GTX115731) antibody at 1:500 dilution.

GTX115731_40401_IHC_18121410_903.jpg

GTX115731 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using MKRN2(GTX115731) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115731_40401_WB_18121410_818.jpg

GTX115731 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
10% SDS PAGE
GTX115731 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169