APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
Not tested in other applications.
Calculated MW
Observed MW
130 kDa. The observed M.W. is based on the following publication. PMID: 24151072
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.65 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human Myosin light chain kinase.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
myosin light chain kinase , AAT7 , KRP , MLCK , MLCK1 , MLCK108 , MLCK210 , MSTP083 , MYLK1 , smMLCK
Cellular Localization
Cytoplasm
Background
This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208](/templates/default/js/lightbox/images/loading.gif "Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208")
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GTX03208 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MLCK antibody [GT1296] (GTX03208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 24151072
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|
|
GTX03208 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MLCK antibody [GT1296] (GTX03208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 24151072
|
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GTX03208 WB Image
WB analysis of various samples using GTX03208 MLCK antibody [GT1296].
Dilution : 1:1000
Loading : 25μg per lane
|
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GTX03208 WB Image
WB analysis of various samples using GTX03208 MLCK antibody [GT1296].
Dilution : 1:1000
Loading : 25μg per lane
|
REFERENCE
There are currently no references for MLCK antibody [GT1296] (GTX03208). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MLCK antibody [GT1296] (GTX03208). Be the first to share your experience with this product.
![Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208](/upload/website/prouct_img/small/GTX03208/GTX03208_4000000842_20210716_WB_w_23053123_329.webp "Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208")
![Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208](/upload/website/prouct_img/small/GTX03208/GTX03208_4000000842_20210716_WB_2_w_23053123_259.webp "Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208")
![Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208](/upload/website/prouct_img/small/GTX03208/GTX03208_25_WB_w_23053123_889.webp "Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208")
![Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208](/upload/website/prouct_img/small/GTX03208/GTX03208_26_WB_w_23053123_833.webp "Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208")
![Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208](/upload/website/prouct_img/normal/GTX03208/GTX03208_4000000842_20210716_WB_w_23053123_329.webp "Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208")
![Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208](/upload/website/prouct_img/normal/GTX03208/GTX03208_4000000842_20210716_WB_2_w_23053123_259.webp "Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208")
![Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208](/upload/website/prouct_img/normal/GTX03208/GTX03208_25_WB_w_23053123_889.webp "Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208")
![Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208](/upload/website/prouct_img/normal/GTX03208/GTX03208_26_WB_w_23053123_833.webp "Anti-MLCK antibody [GT1296] used in Western Blot (WB). GTX03208")