GeneTex
United States (US)

MLCK antibody

GTX64416_20180530_WB_18121411_615.jpg
GTX64416_20180530_IHC-P_2_18121411_513.jpg
GTX64416_20180530_IHC-P_1_18121411_955.jpg
GTX64416_20180530_IHC-P_18121411_850.jpg

Cat No. GTX64416

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

211 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1765-1914 of human MYLK (NP_444253.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SMMLCK Antibody , AAT7 Antibody , MSTP083 Antibody , KRP Antibody , MLCK108 Antibody , MLCK210 Antibody , MYLK Antibody , MLCK1 Antibody , MYLK1 Antibody

Background

This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX64416_20180530_WB_18121411_615.jpg

GTX64416 WB Image

WB analysis of various samples using GTX64416 MLCK antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX64416_20180530_IHC-P_2_18121411_513.jpg

GTX64416 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX64416 MLCK antibody.
Dilution : 1:100

GTX64416_20180530_IHC-P_1_18121411_955.jpg

GTX64416 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX64416 MLCK antibody.
Dilution : 1:100

GTX64416_20180530_IHC-P_18121411_850.jpg

GTX64416 IHC-P Image

IHC-P analysis of human stomach tissue using GTX64416 MLCK antibody.
Dilution : 1:100

Package List Price ($)
$ 319