MLCK (phospho Tyr464) antibody

Cat. No. GTX03352

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ELISA
Reactivity	Human
Package	100 μl ($419)

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Datasheet

View product citations for antibody GTX03352 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:3000
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

211 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from Human MYLK(Accession Q15746), corresponding to amino acid residues L449-L474.

Purification

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin light chain kinase , AAT7 , KRP , MLCK , MLCK1 , MLCK108 , MLCK210 , MSTP083 , MYLK1 , smMLCK

Cellular Localization

Cytoplasm

Background

This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008]

Database

Gene ID: 4638 MYLK
UniProt: Q15746 MYLK

Research Area

DATA IMAGES

GTX03352 WB Image

WB analysis of TNF-α treated A2780 whole cell lysates using GTX03352 MLCK (phospho Tyr464) antibody. -/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).

GTX03352 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX03352 MLCK (phospho Tyr464) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 419

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