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MLH1 antibody [4C9C7]

Anti-MLH1 antibody [4C9C7] used in Western Blot (WB). GTX83336
Anti-MLH1 antibody [4C9C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83336
Anti-MLH1 antibody [4C9C7] used in IHC (Paraffin sections) (IHC-P). GTX83336

Cat No. GTX83336

Host

Mouse

Clonality

Monoclonal

Clone Name

4C9C7

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Monkey
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mutL homolog 1 , COCA2 , FCC2 , HNPCC , HNPCC2 , hMLH1

Cellular Localization

Nucleus

Background

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-MLH1 antibody [4C9C7] used in Western Blot (WB). GTX83336

GTX83336 WB Image

WB analysis of HeLa (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate using GTX83336 MLH1 antibody [4C9C7].

Anti-MLH1 antibody [4C9C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83336

GTX83336 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX83336 MLH1 antibody [4C9C7].
Green : MLH1
Red: Actin filaments

Anti-MLH1 antibody [4C9C7] used in IHC (Paraffin sections) (IHC-P). GTX83336

GTX83336 IHC-P Image

IHC-P analysis of human rectum cancer (left) and ovarian cancer (right) tissue using GTX83336 MLH1 antibody [4C9C7].

REFERENCE

There are currently no references for MLH1 antibody [4C9C7] (GTX83336). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for MLH1 antibody [4C9C7] (GTX83336). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 349