GeneTex
  • Country / Location Selection

United States (US)

MLH1 antibody [G168-728]

Cat No. GTX01744

Host

Mouse

Clonality

Monoclonal

Clone Name

G168-728

Isotype

IgG1

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat, Hamster
Package
500 μl ($389)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1:25-1:100
IP Assay dependent

Note :

IHC-P
Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended.

Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

Colon carcinoma

PROPERTIES

Form

Liquid

Buffer

Tris-HCl buffer, 0.2% BSA, < 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant human MLH-1

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mutL homolog 1 , COCA2 , FCC2 , HNPCC , HNPCC2 , hMLH1

Cellular Localization

Nucleus

Background

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]

Database

Research Area

REFERENCE

REVIEW

There are currently no reviews for MLH1 antibody [G168-728] (GTX01744). Be the first to share your experience with this product.
Package List Price ($)
$ 389