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MLH1 antibody [N1N3]

Overexpression
Anti-MLH1 antibody [N1N3] used in Western Blot (WB). GTX112984
Anti-MLH1 antibody [N1N3] used in Western Blot (WB). GTX112984

Cat. No. GTX112984

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MLH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

Raji , human MLH1-transfected 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MLH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177219

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mutL homolog 1 , COCA2 , FCC2 , HNPCC , HNPCC2 , hMLH1

Cellular Localization

Nucleus

Background

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MLH1 antibody [N1N3] used in Western Blot (WB). GTX112984

GTX112984 WB Image

Overexpression

MLH1 antibody detects MLH1 protein by western blot analysis. Non-transfected (-) and MLH1-transfected (+, ) 293T whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MLH1 antibody (GTX112984) at a dilution of 1:1000.

Anti-MLH1 antibody [N1N3] used in Western Blot (WB). GTX112984

GTX112984 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX112984 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169