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MLH1 antibody [N1N3]

Anti-MLH1 antibody [N1N3] used in Western Blot (WB). GTX112984
Anti-MLH1 antibody [N1N3] used in Western Blot (WB). GTX112984

Cat No. GTX112984

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

Raji , human MLH1-transfected 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MLH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177219

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mutL homolog 1 , COCA2 , FCC2 , HNPCC , HNPCC2 , hMLH1

Cellular Localization

Nucleus

Background

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MLH1 antibody [N1N3] used in Western Blot (WB). GTX112984

GTX112984 WB Image

MLH1 antibody detects MLH1 protein by western blot analysis. Non-transfected (-) and MLH1-transfected (+, ) 293T whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MLH1 antibody (GTX112984) at a dilution of 1:1000.

Anti-MLH1 antibody [N1N3] used in Western Blot (WB). GTX112984

GTX112984 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX112984 diluted at 1:500

REFERENCE

There are currently no references for MLH1 antibody [N1N3] (GTX112984). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for MLH1 antibody [N1N3] (GTX112984). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169