GeneTex
United States (US)

MLH1 antibody [N1N3]

Overexpression
GTX112984_40604_20150508_WB_B_18121410_834.jpg
GTX112984_40604_WB_18121410_395.jpg

Cat No. GTX112984

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

Raji , human MLH1-transfected 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MLH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mutl Homolog 1,Coca2,Fcc2,Hnpcc,Hnpcc2,Hmlh1,Mlh1

Cellular Localization

Nucleus

Background

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX112984_40604_20150508_WB_B_18121410_834.jpg

GTX112984 WB Image

Overexpression

MLH1 antibody detects MLH1 protein by western blot analysis. Non-transfected (-) and MLH1-transfected (+, ) 293T whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MLH1 antibody (GTX112984) at a dilution of 1:1000.

GTX112984_40604_WB_18121410_395.jpg

GTX112984 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX112984 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf