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MLH1 antibody [SP08-04]

Recombinant antibody
Anti-MLH1 antibody [SP08-04] used in Western Blot (WB). GTX01140
Anti-MLH1 antibody [SP08-04] used in Flow cytometry (FACS). GTX01140

Cat. No. GTX01140

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 SP08-04

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human
Package
100 μl ($399)
See all MLH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mutL homolog 1 , COCA2 , FCC2 , HNPCC , HNPCC2 , MLH1 , hMLH1

Cellular Localization

Nucleus

Background

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-MLH1 antibody [SP08-04] used in Western Blot (WB). GTX01140

GTX01140 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MLH1 antibody [SP08-04] (GTX01140) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MLH1 antibody [SP08-04] used in Flow cytometry (FACS). GTX01140

GTX01140 FACS Image

FACS analysis of HeLa cells using GTX01140 MLH1 antibody [SP08-04].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399