GeneTex
United States (US)

MLH1 antibody

Overexpression
GTX101160_40254_IFA_19011506_257.jpg
GTX101160_40254_20150515_WB_B_19011506_176.jpg
GTX101160_40254_WB_19011506_698.jpg

Cat No. GTX101160

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

A549 , H1299 , human MLH1-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MLH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

COCA2 antibody, FCC2 antibody, HNPCC antibody, HNPCC2 antibody, MGC5172 antibody, hMLH1 antibody, MLH1 antibody, DNA mismatch repair protein Mlh1 antibody, mutL protein homolog 1 antibody, mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) antibody

Cellular Localization

Nucleus

Background

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101160_40254_IFA_19011506_257.jpg

GTX101160 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using MLH1(GTX101160) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101160_40254_20150515_WB_B_19011506_176.jpg

GTX101160 WB Image

Overexpression

MLH1 antibody detects MLH1 protein by western blot analysis. Non-transfected (-) and MLH1-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MLH1 antibody (GTX101160) at a dilution of 1:20000.

GTX101160_40254_WB_19011506_698.jpg

GTX101160 WB Image

MLH1 antibody detects MLH1 protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
7.5 % SDS-PAGE
MLH1 antibody (GTX101160) dilution: 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf