*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A549 , H1299 , human MLH1-transfected 293T
Mouse, Rat, Bovine, Pig(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Recombinant protein encompassing a sequence within the center region of human MLH1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
COCA2 antibody, FCC2 antibody, HNPCC antibody, HNPCC2 antibody, MGC5172 antibody, hMLH1 antibody, MLH1 antibody, DNA mismatch repair protein Mlh1 antibody, mutL protein homolog 1 antibody, mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) antibody
This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. [provided by RefSeq]
GTX101160 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using MLH1(GTX101160) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
GTX101160 WB Image
MLH1 antibody detects MLH1 protein by western blot analysis. Non-transfected (-) and MLH1-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MLH1 antibody (GTX101160) at a dilution of 1:20000.
GTX101160 WB Image
MLH1 antibody detects MLH1 protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
7.5 % SDS-PAGE
MLH1 antibody (GTX101160) dilution: 1:500