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MLKL antibody [GT1191]

Recombinant antibody KO/KD
Anti-MLKL antibody [GT1191] used in Western Blot (WB). GTX00953
Anti-MLKL antibody [GT1191] used in Western Blot (WB). GTX00953
Anti-MLKL antibody [GT1191] used in Western Blot (WB). GTX00953

Cat. No. GTX00953

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1191

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all MLKL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human MLKL.

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mixed lineage kinase domain like pseudokinase , MLKL , hMLKL , mixed lineage kinase domain like

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-MLKL antibody [GT1191] used in Western Blot (WB). GTX00953

GTX00953 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MLKL antibody [GT1191] (GTX00953) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MLKL antibody [GT1191] used in Western Blot (WB). GTX00953

GTX00953 WB Image

KO/KD

WB analysis of wild-type (WT) and MLKL knockout (KO) HeLa cell extracts using GTX00953 MLKL antibody [GT1191].
Dilution : 1:1000
Laoding : 25μg

Anti-MLKL antibody [GT1191] used in Western Blot (WB). GTX00953

GTX00953 WB Image

WB analysis of various samples using GTX00953 MLKL antibody [GT1191].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399