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MLKL antibody [HL1849]

Recombinant antibody
Anti-MLKL antibody [HL1849] used in Western Blot (WB). GTX637574
Anti-MLKL antibody [HL1849] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637574

Cat. No. GTX637574

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1849

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MLKL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human MLKL

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mixed lineage kinase domain like pseudokinase , hMLKL

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-MLKL antibody [HL1849] used in Western Blot (WB). GTX637574

GTX637574 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MLKL antibody [HL1849] (GTX637574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MLKL antibody [HL1849] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637574

GTX637574 ICC/IF Image

MLKL antibody [HL1849] detects MLKL protein at cell membrane by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MLKL stained by MLKL antibody [HL1849] (GTX637574) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

H Cui et al. Research Square 2023; TNF-α promotes osteocyte necroptosis by upregulating TLR4 in postmenopausal osteoporosis
Application : IHC-P
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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations