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MLKL antibody

Anti-MLKL antibody used in Western Blot (WB). GTX04591
Anti-MLKL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04591

Cat. No. GTX04591

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all MLKL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% glycerol

Preservative

0.01% thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 369-471 of human MLKL (NP_689862.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mixed lineage kinase domain like pseudokinase , hMLKL

Cellular Localization

Cytoplasm , Cell membrane

Background

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-MLKL antibody used in Western Blot (WB). GTX04591

GTX04591 WB Image

WB analysis of various samples using GTX04591 MLKL antibody.
Loading : 25 μg
Dilution : 1:1000

Anti-MLKL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04591

GTX04591 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX04591 MLKL antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399