GeneTex
United States (US)

MLX antibody

Mlx-antibody-GTX87667-WB-1_18121411_771.jpg

Cat No. GTX87667

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:10000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

33 kDa. ( Note )

Specificity/Sensitivity

Mlx Antibody detects endogenous levels of total Mlx protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Mlx.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mlx, Max Dimerization Protein,Mad7,Mxd7,Tcfl4,Tf4,Bhlhd13,Mlx

Cellular Localization

Found predominantly in the cytoplasm. Found predominantly in the cytoplasm. Found predominantly in the nucleus.

Background

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.

Database

Research Area

DATA IMAGES
Mlx-antibody-GTX87667-WB-1_18121411_771.jpg

GTX87667 WB Image

Western blot analysis of extracts from LOVO cells, using Mlx Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339