GeneTex
United States (US)

MMACHC antibody [N1C2]

GTX106822_39981_IHC_19011506_944.jpg
GTX106822_39981_IHC_M_19011506_213.jpg
GTX106822_WB_19011506_609.jpg
GTX106822_39981_WB_M_brain_19011506_550.jpg
GTX106822_IFA_19011506_658.jpg

Cat No. GTX106822

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Raji , mouse brain

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMACHC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp564I122 antibody, FLJ25671 antibody, RP11-291L19.3 antibody, cblC antibody, MMACHC antibody, methylmalonic aciduria and homocystinuria type C protein antibody, "methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria antibody"

Background

The exact function of the protein encoded by this gene is not known, however, its C-terminal region shows similarity to TonB, a bacterial protein involved in energy transduction for cobalamin (vitamin B12) uptake. Hence, it is postulated that this protein may have a role in the binding and intracellular trafficking of cobalamin. Mutations in this gene are associated with methylmalonic aciduria and homocystinuria type cblC. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX106822_39981_IHC_19011506_944.jpg

GTX106822 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using MMACHC(GTX106822) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106822_39981_IHC_M_19011506_213.jpg

GTX106822 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using MMACHC(GTX106822) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106822_WB_19011506_609.jpg

GTX106822 WB Image

Sample(30 ug whole cell lysate)
A:293T
B:Raji (GTX27908)
12% SDS PAGE
GTX106822 diluted at 1:1000

GTX106822_39981_WB_M_brain_19011506_550.jpg

GTX106822 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX106822 diluted at 1:1000

GTX106822_IFA_19011506_658.jpg

GTX106822 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MMACHC(GTX106822) antibody at 1:100 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf