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MMACHC antibody [N1C2]

Anti-MMACHC antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX106822
Anti-MMACHC antibody [N1C2] used in Western Blot (WB). GTX106822
Anti-MMACHC antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX106822
Anti-MMACHC antibody [N1C2] used in Western Blot (WB). GTX106822
Anti-MMACHC antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106822

Cat. No. GTX106822

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MMACHC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMACHC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950925

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

metabolism of cobalamin associated C , cblC

Background

The exact function of the protein encoded by this gene is not known, however, its C-terminal region shows similarity to TonB, a bacterial protein involved in energy transduction for cobalamin (vitamin B12) uptake. Hence, it is postulated that this protein may have a role in the binding and intracellular trafficking of cobalamin. Mutations in this gene are associated with methylmalonic aciduria and homocystinuria type cblC. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MMACHC antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX106822

GTX106822 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using MMACHC(GTX106822) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MMACHC antibody [N1C2] used in Western Blot (WB). GTX106822

GTX106822 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MMACHC antibody [N1C2] (GTX106822) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 23825108

Anti-MMACHC antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX106822

GTX106822 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using MMACHC(GTX106822) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MMACHC antibody [N1C2] used in Western Blot (WB). GTX106822

GTX106822 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MMACHC antibody [N1C2] (GTX106822) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 23825108

Anti-MMACHC antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106822

GTX106822 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MMACHC(GTX106822) antibody at 1:100 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169