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MMP1 antibody [6A5]

Anti-MMP1 antibody [6A5] used in ELISA (ELISA). GTX60467
Anti-MMP1 antibody [6A5] used in Western Blot (WB). GTX60467
Anti-MMP1 antibody [6A5] used in IHC (Paraffin sections) (IHC-P). GTX60467
Anti-MMP1 antibody [6A5] used in Flow cytometry (FACS). GTX60467
Anti-MMP1 antibody [6A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60467

Cat. No. GTX60467

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6A5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all MMP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human MMP1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 1 , CLG , CLGN

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-MMP1 antibody [6A5] used in ELISA (ELISA). GTX60467

GTX60467 ELISA Image

ELISA analysis of antigen using GTX60467 MMP1 antibody [6A5].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-MMP1 antibody [6A5] used in Western Blot (WB). GTX60467

GTX60467 WB Image

WB analysis of HEK293 (1) and MMP1(AA: 24-213)-hIgGFc transfected HEK293 (2) cell lysate using GTX60467 MMP1 antibody [6A5].

Anti-MMP1 antibody [6A5] used in IHC (Paraffin sections) (IHC-P). GTX60467

GTX60467 IHC-P Image

IHC-P analysis of human cervical cancer tissue (left) and human kidney cancer tissue (right) using GTX60467 MMP1 antibody [6A5].

Anti-MMP1 antibody [6A5] used in Flow cytometry (FACS). GTX60467

GTX60467 FACS Image

FACS analysis of HeLa cells using GTX60467 MMP1 antibody [6A5].
Green : MMP1
Purple : negative control

Anti-MMP1 antibody [6A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60467

GTX60467 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60467 MMP1 antibody [6A5].
Green : MMP1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409