MMP1 antibody [HL1259]

Cat. No. GTX636661

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1259
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all MMP1 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of Human MMP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910033

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 1 , CLG , CLGN

Background

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Database

Gene ID: 4312 MMP1
UniProt: P03956 MMP1

Research Area

DATA IMAGES

GTX636661 ICC/IF Image

MMP1 antibody [HL1259] detects MMP1 protein by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MMP1 stained by MMP1 antibody [HL1259] (GTX636661) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636661 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP1 antibody [HL1259] (GTX636661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636661 IHC-P Image

MMP1 antibody [HL1259] detects MMP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
MMP1 stained by MMP1 antibody [HL1259] (GTX636661) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations