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MMP1 antibody

Anti-MMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00674
Anti-MMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX00674
Anti-MMP1 antibody used in Western Blot (WB). GTX00674

Cat. No. GTX00674

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MMP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 1 , CLG , CLGN

Background

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-MMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00674

GTX00674 ICC/IF Image

ICC/IF analysis of formalin-fixed MCF7 cells using GTX00674 MMP1 antibody.
Red : Primary antibody
Blue : DAPI
Perrmeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-MMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX00674

GTX00674 IHC-P Image

IHC-P analysis of human lung tissue cancer using GTX00674 MMP1 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-MMP1 antibody used in Western Blot (WB). GTX00674

GTX00674 WB Image

WB analysis of various samples using GTX00674 MMP1 antibody.
A : HeLa whole cell lysate
B : MCF7 whole cell lysate
C : MDA-MB-435 whole cell lysate
D : Raw264.7 whole cell lysate
E : PC12 whole cell lysate
F : NIH3T3 whole cell lysate

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399