Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP1 antibody (GTX100534) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MMP1 antibody detects secreted MMP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
MMP1 stained by MMP1 antibody (GTX100534) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MMP1 antibody detects MMP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MMP1 stained by MMP1 antibody (GTX100534) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

MMP1 antibody detects MMP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
MMP1 stained by MMP1 antibody (GTX100534) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MMP1 antibody detects MMP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
MMP1 stained by MMP1 antibody (GTX100534) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the journal Aging Dis in 2019. PMID: 31164994

The data was published in the journal Nat Commun in 2017.PMID: 28489070