GeneTex
United States (US)

MMP1 (cleaved Phe100) antibody

MMP1-cleaved-Phe100-antibody-GTX86957-WB-1_18121411_238.jpg

Cat No. GTX86957

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:10000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

19 kDa. ( Note )

Specificity/Sensitivity

MMP1 (Cleaved-Phe100) Antibody detects endogenous levels of fragment of activated MMP1 resulting from cleavage adjacent to Phe100.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MMP1.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 1,Clg,Clgn,Mmp1

Background

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

Database

Research Area

DATA IMAGES
MMP1-cleaved-Phe100-antibody-GTX86957-WB-1_18121411_238.jpg

GTX86957 WB Image

Western blot analysis of extracts from A549 cells, treated with etoposide 25uM 24h, using MMP1 (Cleaved-Phe100) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339