GeneTex
United States (US)

MMP10 antibody

Overexpression
GTX103287_40317_20150924_WB_H_plasma_19011506_947.jpg
GTX103287_40317_20141231_IHC_19011506_901.jpg
GTX103287_40317_20150813_WB_B_19011506_401.jpg

Cat No. GTX103287

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

human plasma , human MMP10 -transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 10,Sl-2,Stmy2,Mmp10

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]

Database

DATA IMAGES
GTX103287_40317_20150924_WB_H_plasma_19011506_947.jpg

GTX103287 WB Image

MMP10 antibody detects MMP10 protein by western blot analysis. Human tissue extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP10 antibody (GTX103287) diluted at 1:2000.

GTX103287_40317_20141231_IHC_19011506_901.jpg

GTX103287 IHC-P Image

MMP10 antibody detects MMP10 protein at secreted on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
MMP10 antibody (GTX103287) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103287_40317_20150813_WB_B_19011506_401.jpg

GTX103287 WB Image

Overexpression

MMP10 antibody detects MMP10 protein by western blot analysis. Non-transfected (-) and MMP10-transfected (+) whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP10 antibody (GTX103287) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169