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MMP10 antibody

Overexpression
Anti-MMP10 antibody used in IHC (Paraffin sections) (IHC-P). GTX103287
Anti-MMP10 antibody used in Western Blot (WB). GTX103287
Anti-MMP10 antibody used in Western Blot (WB). GTX103287

Cat. No. GTX103287

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MMP10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

human MMP10-transfected 293T , human plasma

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950927

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 10 , SL-2 , STMY2

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MMP10 antibody used in IHC (Paraffin sections) (IHC-P). GTX103287

GTX103287 IHC-P Image

MMP10 antibody detects MMP10 protein at secreted on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
MMP10 antibody (GTX103287) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MMP10 antibody used in Western Blot (WB). GTX103287

GTX103287 WB Image

MMP10 antibody detects MMP10 protein by western blot analysis. Human tissue extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP10 antibody (GTX103287) diluted at 1:2000.

Anti-MMP10 antibody used in Western Blot (WB). GTX103287

GTX103287 WB Image

Overexpression

MMP10 antibody detects MMP10 protein by western blot analysis. Non-transfected (-) and MMP10-transfected (+) whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP10 antibody (GTX103287) diluted at 1:1000.

REFERENCE

Application Reference

Nakagawa M et al. Arch Oral Biol 2021; 123 : 105035 Long-term exposure to butyric acid induces excessive production of matrix metalloproteases in human gingival fibroblasts.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169