GeneTex
United States (US)

MMP12 (cleaved Glu106) antibody

MMP12-cleaved-Glu106-antibody-GTX86934-WB-1_18121411_625.jpg

Cat No. GTX86934

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

42 kDa. ( Note )

Specificity/Sensitivity

MMP12 (Cleaved-Glu106) Antibody detects endogenous levels of fragment of activated MMP12 resulting from cleavage adjacent to Glu106.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MMP12.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 12,Hme,Me,Mme,Mmp-12,Mmp12

Background

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

Database

Research Area

DATA IMAGES
MMP12-cleaved-Glu106-antibody-GTX86934-WB-1_18121411_625.jpg

GTX86934 WB Image

Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide 25uM 1h, using MMP12 (Cleaved-Glu106) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339