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MMP13 antibody [N3C1], Internal

Orthogonal
Anti-MMP13 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100665
Anti-MMP13 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100665
Anti-MMP13 antibody [N3C1], Internal used in Western Blot (WB). GTX100665
Anti-MMP13 antibody [N3C1], Internal used in Western Blot (WB). GTX100665
Anti-MMP13 antibody [N3C1], Internal used in Western Blot (WB). GTX100665

Cat. No. GTX100665

Reference 18
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MMP13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Rabbit, Sheep, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MMP13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037446

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 13 , CLG3 , MANDP1 , MDST , MMP-13

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MMP13 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100665

GTX100665 ICC/IF Image

MMP13 antibody [N3C1], Internal detects MMP13 protein at cytoplasm by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: MMP13 protein stained by MMP13 antibody [N3C1], Internal (GTX100665) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-MMP13 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100665

GTX100665 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using MMP13(GTX100665) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MMP13 antibody [N3C1], Internal used in Western Blot (WB). GTX100665

GTX100665 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP13 antibody [N3C1], Internal (GTX100665) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-MMP13 antibody [N3C1], Internal used in Western Blot (WB). GTX100665

GTX100665 WB Image

Orthogonal

The data was published in the journal PLoS One in 2017. PMID: 28604807

Anti-MMP13 antibody [N3C1], Internal used in Western Blot (WB). GTX100665

GTX100665 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28604807

REFERENCE

Application Reference

Chen MF et al. Biomedicines 2023; 11 (4) Characterization and Advancement of an Evaluation Method for the Treatment of Spontaneous Osteoarthritis in STR/ort Mice: GRGDS Peptides as a Potential Treatment for Osteoarthritis.
Tan Y et al. Pharmaceuticals (Basel) 2023; 16 (3) FOXO3-Activated circFGFBP1 Inhibits Extracellular Matrix Degradation and Nucleus Pulposus Cell Death via miR-9-5p/BMP2 Axis in Intervertebral Disc Degeneration In Vivo and In Vitro.
Application : IHC-Fr
Reactivity : Rat
Hsueh YH et al. J Orthop Translat 2023; 38 : 141-155 Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
Chen MF et al. Bone Joint Res 2023; 12 (12) : 734-746 The role of EDIL3 in maintaining cartilage extracellular matrix and inhibiting osteoarthritis development.
Yang G et al. Front Pharmacol 2022; 13 : 963506 Celastrol ameliorates osteoarthritis <i>via</i> regulating TLR2/NF-κB signaling pathway.
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Submit a Reference

REVIEW

MMP13 antibody [N3C1], Internal Cat. No. GTX100665

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-MMP13 antibody [N3C1], Internal used in Western Blot (WB). GTX100665
Date : Anonymous submitted on 22-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : SW1353 cells
Amount used : 25μg
Blocking : Block Ace, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Figure Note : IL-1β (10ng/ml, 24h) upregulates matrix metalloproteinase-13 (MMP-13).
Anti-MMP13 antibody [N3C1], Internal used in Western Blot (WB). GTX100665
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : SCC4 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM ADC treatment for 24h.
Anti-MMP13 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100665
Date : Anonymous submitted on 10-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Embryonic mouse bone
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : Tris-EDTA pH9, boil°C, 0.5Hr
Primary Antibody : 1:100, 4°C, 16Hr
Submit a Review
Application Reference
Chen MF et al. Biomedicines 2023; 11 (4) Characterization and Advancement of an Evaluation Method for the Treatment of Spontaneous Osteoarthritis in STR/ort Mice: GRGDS Peptides as a Potential Treatment for Osteoarthritis.
Tan Y et al. Pharmaceuticals (Basel) 2023; 16 (3) FOXO3-Activated circFGFBP1 Inhibits Extracellular Matrix Degradation and Nucleus Pulposus Cell Death via miR-9-5p/BMP2 Axis in Intervertebral Disc Degeneration In Vivo and In Vitro.
Application : IHC-Fr
Reactivity : Rat
Hsueh YH et al. J Orthop Translat 2023; 38:141-155 Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
Chen MF et al. Bone Joint Res 2023; 12 (12):734-746 The role of EDIL3 in maintaining cartilage extracellular matrix and inhibiting osteoarthritis development.
Yang G et al. Front Pharmacol 2022; 13:963506 Celastrol ameliorates osteoarthritis <i>via</i> regulating TLR2/NF-κB signaling pathway.
Lecheng Zhang et al. Cell Cycle 2021; 20 (11):1052-1066 Knockdown of hsa_circ_0134111 alleviates the symptom of osteoarthritis via sponging microRNA-224-5p.
Application : ICC/IF
Reactivity : Human
Kai-Chieh Chou et al. Polymers (Basel) 2021; 13 (20) Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model.
Application : IHC-P
Reactivity : Rat
Yen YT et al. Commun Biol 2021; 4 (1):658 PP2A in LepR+ mesenchymal stem cells contributes to embryonic and postnatal endochondral ossification through Runx2 dephosphorylation.
Application : WB, IHC-P
Reactivity : Mouse
Wang SW et al. J Cell Physiol 2020; (Epub) Melatonin impedes prostate cancer metastasis by suppressing MMP-13 expression.
Application : WB
Reactivity : Human
Shao LT et al. Bone Joint Res 2020; 9 (10):675-688 Parathyroid hormone (1-34) ameliorates cartilage degeneration and subchondral bone deterioration in collagenase-induced osteoarthritis model in mice.
Application : IHC-P
Reactivity : Mouse
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
Application : WB
Reactivity : Human
Nakagawa Y et al. J Orthop Res 2018; (Epub) Duration of postoperative immobilization affects MMP activity at the healing graft-bone interface: Evaluation in a mouse ACL reconstruction model.
Application : IHC-P
Reactivity : Mouse
Fang CC et al. Sci Rep 2018; 8 (1):13802 AAV serotype 8-mediated liver specific GNMT expression delays progression of hepatocellular carcinoma and prevents carbon tetrachloride-induced liver damage.
Application : IHC-P
Reactivity : Mouse
Hsu SJ et al. Clin Sci (Lond) 2018; (Epub) Extrahepatic angiogenesis hinders recovery of portal hypertension and collaterals in rats with cirrhosis resolution.
Application : WB
Reactivity : Rat
Lin KT et al. Am J Chin Med 2018;:43116 Rhodiola crenulata Attenuates γ-Ray Induced Cellular Injury Via Modulation of Oxidative Stress in Human Skin Cells.
Application : WB
Reactivity : Human
Tsai YF et al. PLoS One 2017; 12 (6):e0178173 Brain-derived neurotrophic factor (BDNF) -TrKB signaling modulates cancer-endothelial cells interaction and affects the outcomes of triple negative breast cancer.
Application : WB
Reactivity : Human
Qu J et al. Clin Exp Pharmacol Physiol 2015; (Epub) PFKFB3 modulates glycolytic metabolism and alleviates endoplasmic reticulum stress in human osteoarthritis cartilage.
Application : WB
Reactivity : Human
Nakayama S et al. Medicine (Baltimore) 2015; 94 (22):e866 Dermatopathic Lymphadenopathy With Increased IgG4-Positive Plasma Cells.
Application : IHC
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169