Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP13 antibody [N3C1], Internal (GTX100665) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using MMP13(GTX100665) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MMP13 antibody [N3C1], Internal detects MMP13 protein at cytoplasm by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: MMP13 protein stained by MMP13 antibody [N3C1], Internal (GTX100665) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

The data was published in the journal PLoS One in 2017. PMID: 28604807

The data was published in the journal PLoS One in 2017. PMID: 28604807