GeneTex
United States (US)

MMP13 antibody [N3C1], Internal

GTX100665_39995_IHC_19011506_609.jpg
GTX100665_39995_20151230_IFA_19011506_325.jpg
GTX100665_WB_19011506_690.jpg

Cat No. GTX100665

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Rabbit(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MMP13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 13,Clg3,Mandp1,Mdst,Mmp-13,Mmp13

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100665_39995_IHC_19011506_609.jpg

GTX100665 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using MMP13(GTX100665) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100665_39995_20151230_IFA_19011506_325.jpg

GTX100665 ICC/IF Image

MMP13 antibody [N3C1], Internal detects MMP13 protein at cytoplasm by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: MMP13 protein stained by MMP13 antibody [N3C1], Internal (GTX100665) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX100665_WB_19011506_690.jpg

GTX100665 WB Image

Sample (30 µg of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX100665 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf