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MMP14 antibody

Anti-MMP14 antibody used in Western Blot (WB). GTX132884
Anti-MMP14 antibody used in Western Blot (WB). GTX132884
Anti-MMP14 antibody used in Western Blot (WB). GTX132884

Cat No. GTX132884

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

U87-MG , Neuro2A , MMP14-transfected 293T

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Human MMP14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

matrix metallopeptidase 14,MMP-14,MMP-X1,MT-MMP,MT-MMP 1,MT1-MMP,MT1MMP,MTMMP1,WNCHRS

Cellular Localization

Membrane; Single-pass type I membrane protein (Potential) , Melanosome

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-MMP14 antibody used in Western Blot (WB). GTX132884

GTX132884 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP14 antibody (GTX132884) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MMP14 antibody used in Western Blot (WB). GTX132884

GTX132884 WB Image

Untreated (–) and treated (+) U87-MG whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP14 antibody (GTX132884) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MMP14 antibody used in Western Blot (WB). GTX132884

GTX132884 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP14 antibody (GTX132884) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169