GeneTex
United States (US)

MMP14 (cleaved Tyr112) antibody

MMP14-cleaved-Tyr112-antibody-GTX86916-WB-1_18121411_580.jpg

Cat No. GTX86916

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

53 kDa. ( Note )

Specificity/Sensitivity

MMP14 (Cleaved-Tyr112) Antibody detects endogenous levels of fragment of activated MMP14 resulting from cleavage adjacent to Tyr112.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MMP14.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Matrix Metallopeptidase 14,Mmp-14,Mmp-X1,Mt-Mmp,Mt-Mmp 1,Mt1-Mmp,Mt1Mmp,Mtmmp1,Wnchrs,Mmp14

Cellular Localization

Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Background

Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.

Database

Research Area

DATA IMAGES
MMP14-cleaved-Tyr112-antibody-GTX86916-WB-1_18121411_580.jpg

GTX86916 WB Image

Western blot analysis of extracts from 293 cells, treated with etoposide 25uM 1h, using MMP14 (Cleaved-Tyr112) Antibody. The lane on the right is treated with the synthesized peptide.